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transwell chambers  (Greiner Bio)


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    Structured Review

    Greiner Bio transwell chambers
    Migration assay using Boyden chambers. Equal numbers of cells were placed in <t>Transwell/Boyden</t> Chambers in serum-free RPMI 1640 medium containing increasing concentrations of Bortezomib (0, 20, 40, and 80 nM), and the inserts were placed in microwells containing FBS-supplemented medium. The cells were left for 24 h to migrate, and afterwards, the cells crossing the filters were fixed and stained with crystal violet. Photographs were taken using a 10× objective and the fixed cells were counted using the multipoint tool by ImageJ. Photographs were taken using a 10× objective, and the fixed cells were counted using the multipoint tool by ImageJ.
    Transwell Chambers, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 97/100, based on 549 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/24+well+plate+culture+chamber/Thincert+Cell+Culture+Insert+For+24+Well+Plates/pmc12110616-16-0-9
    Average 97 stars, based on 549 article reviews
    transwell chambers - by Bioz Stars, 2026-10
    97/100 stars

    Images

    1) Product Images from "The Transcription Axes ERK-Elk1, JNK-cJun, and JAK-STAT Promote Autophagy Activation and Proteasome Inhibitor Resistance in Prostate Cancer Cells"

    Article Title: The Transcription Axes ERK-Elk1, JNK-cJun, and JAK-STAT Promote Autophagy Activation and Proteasome Inhibitor Resistance in Prostate Cancer Cells

    Journal: Current Issues in Molecular Biology

    doi: 10.3390/cimb47050352

    Migration assay using Boyden chambers. Equal numbers of cells were placed in Transwell/Boyden Chambers in serum-free RPMI 1640 medium containing increasing concentrations of Bortezomib (0, 20, 40, and 80 nM), and the inserts were placed in microwells containing FBS-supplemented medium. The cells were left for 24 h to migrate, and afterwards, the cells crossing the filters were fixed and stained with crystal violet. Photographs were taken using a 10× objective and the fixed cells were counted using the multipoint tool by ImageJ. Photographs were taken using a 10× objective, and the fixed cells were counted using the multipoint tool by ImageJ.
    Figure Legend Snippet: Migration assay using Boyden chambers. Equal numbers of cells were placed in Transwell/Boyden Chambers in serum-free RPMI 1640 medium containing increasing concentrations of Bortezomib (0, 20, 40, and 80 nM), and the inserts were placed in microwells containing FBS-supplemented medium. The cells were left for 24 h to migrate, and afterwards, the cells crossing the filters were fixed and stained with crystal violet. Photographs were taken using a 10× objective and the fixed cells were counted using the multipoint tool by ImageJ. Photographs were taken using a 10× objective, and the fixed cells were counted using the multipoint tool by ImageJ.

    Techniques Used: Migration, Staining

    Chemotaxis Assay. Equal numbers of cells were placed in Transwell/Boyden Chambers in serum-free RPMI 1640 medium, and the inserts were placed in microwells with FBS-supplemented medium containing increasing concentrations of Bortezomib (0, 20, 40, and 80 nM) for 24 h. ( a ) The percentage of migrated cells was compared to the naïve untreated sample; and ( b ) the percentages of total migrated cells, compared to each clone’s baseline conditions (untreated for the naïve cells, and 40 nM Bortezomib for the RB40 cells). The results were analyzed using multiple comparisons of one-way ANOVA in the Prism 8 software (“ns” corresponds to non-significant, *** corresponds to a p -value = 0.0001; and **** corresponds to a p -value < 0.0001). The error bars represent the standard error of the mean (SEM).
    Figure Legend Snippet: Chemotaxis Assay. Equal numbers of cells were placed in Transwell/Boyden Chambers in serum-free RPMI 1640 medium, and the inserts were placed in microwells with FBS-supplemented medium containing increasing concentrations of Bortezomib (0, 20, 40, and 80 nM) for 24 h. ( a ) The percentage of migrated cells was compared to the naïve untreated sample; and ( b ) the percentages of total migrated cells, compared to each clone’s baseline conditions (untreated for the naïve cells, and 40 nM Bortezomib for the RB40 cells). The results were analyzed using multiple comparisons of one-way ANOVA in the Prism 8 software (“ns” corresponds to non-significant, *** corresponds to a p -value = 0.0001; and **** corresponds to a p -value < 0.0001). The error bars represent the standard error of the mean (SEM).

    Techniques Used: Chemotaxis Assay, Clone Assay, Software

    Chemotaxis assay using Boyden chambers. Equal numbers of cells were placed in Transwell/Boyden Chambers in serum-free RPMI 1640 medium, and the inserts were placed in microwells with FBS-supplemented medium containing increasing concentrations of Bortezomib (0, 20, 40, and 80 nM). The cells were left for 24 h to migrate, and afterwards, the cells crossing the filters were fixed and stained with crystal violet. Photographs were taken using a 10× objective, and the fixed cells were counted using the multipoint tool by ImageJ.
    Figure Legend Snippet: Chemotaxis assay using Boyden chambers. Equal numbers of cells were placed in Transwell/Boyden Chambers in serum-free RPMI 1640 medium, and the inserts were placed in microwells with FBS-supplemented medium containing increasing concentrations of Bortezomib (0, 20, 40, and 80 nM). The cells were left for 24 h to migrate, and afterwards, the cells crossing the filters were fixed and stained with crystal violet. Photographs were taken using a 10× objective, and the fixed cells were counted using the multipoint tool by ImageJ.

    Techniques Used: Chemotaxis Assay, Staining

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    Image Search Results


    Functional assays of MERTK in cervical cancer cells. ( A ) mRNA levels following MERTK knockdown with siRNA(n=3). ( B ) Assessment of the impact of MERTK knockdown on the proliferation capacity of cervical cancer cells using the MTS assay(n=3). ( C ) Evaluation of the migratory ability of SiHa and HeLa cells following low expression of MERTK through a scratch assay(n=3). ( D ) Assessment of the effects of MERTK silencing on the migratory and invasive capabilities of cervical cancer cells using transwell migration and invasion assays(n=3). Data are presented as mean±SD. (** p < 0.01, *** p < 0.001, **** p < 0.0001).

    Journal: International Journal of Women's Health

    Article Title: The Sensitive Genes for Cervical Cancer: Two-Sample Mendelian Randomization with Experimental Validation

    doi: 10.2147/IJWH.S516444

    Figure Lengend Snippet: Functional assays of MERTK in cervical cancer cells. ( A ) mRNA levels following MERTK knockdown with siRNA(n=3). ( B ) Assessment of the impact of MERTK knockdown on the proliferation capacity of cervical cancer cells using the MTS assay(n=3). ( C ) Evaluation of the migratory ability of SiHa and HeLa cells following low expression of MERTK through a scratch assay(n=3). ( D ) Assessment of the effects of MERTK silencing on the migratory and invasive capabilities of cervical cancer cells using transwell migration and invasion assays(n=3). Data are presented as mean±SD. (** p < 0.01, *** p < 0.001, **** p < 0.0001).

    Article Snippet: Cell migration and invasion assays were conducted using a 24-well cell culture plate with transwell chambers (3422, Corning, USA).

    Techniques: Functional Assay, Knockdown, MTS Assay, Expressing, Wound Healing Assay, Migration

    Functional assays of SERPINF1 in cervical cancer cells. ( A ) Validation of SERPINF1 mRNA levels following siRNA-mediated knockdown(n=3). ( B ) Evaluation of the changes in proliferation capacity of SiHa and HeLa cells after SERPINF1 knockdown using the MTS assay(n=3). ( C ) Assessment of the impact of low SERPINF1 expression on the migratory ability of SiHa and HeLa cells through a scratch assay(n=3). ( D ) Transwell migration and invasion assays of SiHa and HeLa cells with SERPINF1 silencing (n=3). Data are presented as mean±SD. (** p < 0.01, *** p < 0.001, **** p < 0.0001).

    Journal: International Journal of Women's Health

    Article Title: The Sensitive Genes for Cervical Cancer: Two-Sample Mendelian Randomization with Experimental Validation

    doi: 10.2147/IJWH.S516444

    Figure Lengend Snippet: Functional assays of SERPINF1 in cervical cancer cells. ( A ) Validation of SERPINF1 mRNA levels following siRNA-mediated knockdown(n=3). ( B ) Evaluation of the changes in proliferation capacity of SiHa and HeLa cells after SERPINF1 knockdown using the MTS assay(n=3). ( C ) Assessment of the impact of low SERPINF1 expression on the migratory ability of SiHa and HeLa cells through a scratch assay(n=3). ( D ) Transwell migration and invasion assays of SiHa and HeLa cells with SERPINF1 silencing (n=3). Data are presented as mean±SD. (** p < 0.01, *** p < 0.001, **** p < 0.0001).

    Article Snippet: Cell migration and invasion assays were conducted using a 24-well cell culture plate with transwell chambers (3422, Corning, USA).

    Techniques: Functional Assay, Biomarker Discovery, Knockdown, MTS Assay, Expressing, Wound Healing Assay, Migration

    Migration assay using Boyden chambers. Equal numbers of cells were placed in Transwell/Boyden Chambers in serum-free RPMI 1640 medium containing increasing concentrations of Bortezomib (0, 20, 40, and 80 nM), and the inserts were placed in microwells containing FBS-supplemented medium. The cells were left for 24 h to migrate, and afterwards, the cells crossing the filters were fixed and stained with crystal violet. Photographs were taken using a 10× objective and the fixed cells were counted using the multipoint tool by ImageJ. Photographs were taken using a 10× objective, and the fixed cells were counted using the multipoint tool by ImageJ.

    Journal: Current Issues in Molecular Biology

    Article Title: The Transcription Axes ERK-Elk1, JNK-cJun, and JAK-STAT Promote Autophagy Activation and Proteasome Inhibitor Resistance in Prostate Cancer Cells

    doi: 10.3390/cimb47050352

    Figure Lengend Snippet: Migration assay using Boyden chambers. Equal numbers of cells were placed in Transwell/Boyden Chambers in serum-free RPMI 1640 medium containing increasing concentrations of Bortezomib (0, 20, 40, and 80 nM), and the inserts were placed in microwells containing FBS-supplemented medium. The cells were left for 24 h to migrate, and afterwards, the cells crossing the filters were fixed and stained with crystal violet. Photographs were taken using a 10× objective and the fixed cells were counted using the multipoint tool by ImageJ. Photographs were taken using a 10× objective, and the fixed cells were counted using the multipoint tool by ImageJ.

    Article Snippet: Transwell chambers (PET filter, 8 μm pore diameter) , Greiner Bio-One (Kremsmünster, Austria) , 662638.

    Techniques: Migration, Staining

    Chemotaxis Assay. Equal numbers of cells were placed in Transwell/Boyden Chambers in serum-free RPMI 1640 medium, and the inserts were placed in microwells with FBS-supplemented medium containing increasing concentrations of Bortezomib (0, 20, 40, and 80 nM) for 24 h. ( a ) The percentage of migrated cells was compared to the naïve untreated sample; and ( b ) the percentages of total migrated cells, compared to each clone’s baseline conditions (untreated for the naïve cells, and 40 nM Bortezomib for the RB40 cells). The results were analyzed using multiple comparisons of one-way ANOVA in the Prism 8 software (“ns” corresponds to non-significant, *** corresponds to a p -value = 0.0001; and **** corresponds to a p -value < 0.0001). The error bars represent the standard error of the mean (SEM).

    Journal: Current Issues in Molecular Biology

    Article Title: The Transcription Axes ERK-Elk1, JNK-cJun, and JAK-STAT Promote Autophagy Activation and Proteasome Inhibitor Resistance in Prostate Cancer Cells

    doi: 10.3390/cimb47050352

    Figure Lengend Snippet: Chemotaxis Assay. Equal numbers of cells were placed in Transwell/Boyden Chambers in serum-free RPMI 1640 medium, and the inserts were placed in microwells with FBS-supplemented medium containing increasing concentrations of Bortezomib (0, 20, 40, and 80 nM) for 24 h. ( a ) The percentage of migrated cells was compared to the naïve untreated sample; and ( b ) the percentages of total migrated cells, compared to each clone’s baseline conditions (untreated for the naïve cells, and 40 nM Bortezomib for the RB40 cells). The results were analyzed using multiple comparisons of one-way ANOVA in the Prism 8 software (“ns” corresponds to non-significant, *** corresponds to a p -value = 0.0001; and **** corresponds to a p -value < 0.0001). The error bars represent the standard error of the mean (SEM).

    Article Snippet: Transwell chambers (PET filter, 8 μm pore diameter) , Greiner Bio-One (Kremsmünster, Austria) , 662638.

    Techniques: Chemotaxis Assay, Clone Assay, Software

    Chemotaxis assay using Boyden chambers. Equal numbers of cells were placed in Transwell/Boyden Chambers in serum-free RPMI 1640 medium, and the inserts were placed in microwells with FBS-supplemented medium containing increasing concentrations of Bortezomib (0, 20, 40, and 80 nM). The cells were left for 24 h to migrate, and afterwards, the cells crossing the filters were fixed and stained with crystal violet. Photographs were taken using a 10× objective, and the fixed cells were counted using the multipoint tool by ImageJ.

    Journal: Current Issues in Molecular Biology

    Article Title: The Transcription Axes ERK-Elk1, JNK-cJun, and JAK-STAT Promote Autophagy Activation and Proteasome Inhibitor Resistance in Prostate Cancer Cells

    doi: 10.3390/cimb47050352

    Figure Lengend Snippet: Chemotaxis assay using Boyden chambers. Equal numbers of cells were placed in Transwell/Boyden Chambers in serum-free RPMI 1640 medium, and the inserts were placed in microwells with FBS-supplemented medium containing increasing concentrations of Bortezomib (0, 20, 40, and 80 nM). The cells were left for 24 h to migrate, and afterwards, the cells crossing the filters were fixed and stained with crystal violet. Photographs were taken using a 10× objective, and the fixed cells were counted using the multipoint tool by ImageJ.

    Article Snippet: Transwell chambers (PET filter, 8 μm pore diameter) , Greiner Bio-One (Kremsmünster, Austria) , 662638.

    Techniques: Chemotaxis Assay, Staining