Journal: International Journal of Women's Health
Article Title: The Sensitive Genes for Cervical Cancer: Two-Sample Mendelian Randomization with Experimental Validation
doi: 10.2147/IJWH.S516444
Figure Lengend Snippet: Functional assays of SERPINF1 in cervical cancer cells. ( A ) Validation of SERPINF1 mRNA levels following siRNA-mediated knockdown(n=3). ( B ) Evaluation of the changes in proliferation capacity of SiHa and HeLa cells after SERPINF1 knockdown using the MTS assay(n=3). ( C ) Assessment of the impact of low SERPINF1 expression on the migratory ability of SiHa and HeLa cells through a scratch assay(n=3). ( D ) Transwell migration and invasion assays of SiHa and HeLa cells with SERPINF1 silencing (n=3). Data are presented as mean±SD. (** p < 0.01, *** p < 0.001, **** p < 0.0001).
Article Snippet: Cell migration and invasion assays were conducted using a 24-well cell culture plate with transwell chambers (3422, Corning, USA).
Techniques: Functional Assay, Biomarker Discovery, Knockdown, MTS Assay, Expressing, Wound Healing Assay, Migration