transwell chambers (Greiner Bio)
Structured Review

Transwell Chambers, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 97/100, based on 549 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/24+well+plate+culture+chamber/Thincert+Cell+Culture+Insert+For+24+Well+Plates/pmc12110616-16-0-9
Average 97 stars, based on 549 article reviews
Images
1) Product Images from "The Transcription Axes ERK-Elk1, JNK-cJun, and JAK-STAT Promote Autophagy Activation and Proteasome Inhibitor Resistance in Prostate Cancer Cells"
Article Title: The Transcription Axes ERK-Elk1, JNK-cJun, and JAK-STAT Promote Autophagy Activation and Proteasome Inhibitor Resistance in Prostate Cancer Cells
Journal: Current Issues in Molecular Biology
doi: 10.3390/cimb47050352
Figure Legend Snippet: Migration assay using Boyden chambers. Equal numbers of cells were placed in Transwell/Boyden Chambers in serum-free RPMI 1640 medium containing increasing concentrations of Bortezomib (0, 20, 40, and 80 nM), and the inserts were placed in microwells containing FBS-supplemented medium. The cells were left for 24 h to migrate, and afterwards, the cells crossing the filters were fixed and stained with crystal violet. Photographs were taken using a 10× objective and the fixed cells were counted using the multipoint tool by ImageJ. Photographs were taken using a 10× objective, and the fixed cells were counted using the multipoint tool by ImageJ.
Techniques Used: Migration, Staining
Figure Legend Snippet: Chemotaxis Assay. Equal numbers of cells were placed in Transwell/Boyden Chambers in serum-free RPMI 1640 medium, and the inserts were placed in microwells with FBS-supplemented medium containing increasing concentrations of Bortezomib (0, 20, 40, and 80 nM) for 24 h. ( a ) The percentage of migrated cells was compared to the naïve untreated sample; and ( b ) the percentages of total migrated cells, compared to each clone’s baseline conditions (untreated for the naïve cells, and 40 nM Bortezomib for the RB40 cells). The results were analyzed using multiple comparisons of one-way ANOVA in the Prism 8 software (“ns” corresponds to non-significant, *** corresponds to a p -value = 0.0001; and **** corresponds to a p -value < 0.0001). The error bars represent the standard error of the mean (SEM).
Techniques Used: Chemotaxis Assay, Clone Assay, Software
Figure Legend Snippet: Chemotaxis assay using Boyden chambers. Equal numbers of cells were placed in Transwell/Boyden Chambers in serum-free RPMI 1640 medium, and the inserts were placed in microwells with FBS-supplemented medium containing increasing concentrations of Bortezomib (0, 20, 40, and 80 nM). The cells were left for 24 h to migrate, and afterwards, the cells crossing the filters were fixed and stained with crystal violet. Photographs were taken using a 10× objective, and the fixed cells were counted using the multipoint tool by ImageJ.
Techniques Used: Chemotaxis Assay, Staining
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